Mouse thbs1
Nettet10. jun. 2008 · 188060 - THROMBOSPONDIN I; THBS1 - TSP1 - THBS1 To explore the function of thrombospondin I in vivo, Lawler et al. (1998) disrupted the Thbs1 gene by homologous recombination in the mouse genome. Platelets from these mice were completely deficient in Thbs1 protein; however, thrombin-induced platelet aggregation … NettetView/Edit Mouse. Thrombospondin 1, abbreviated as THBS1, is a protein that in humans is encoded by the THBS1 gene. [5] [6] Thrombospondin 1 is a subunit of a disulfide …
Mouse thbs1
Did you know?
Nettet18. sep. 2013 · Exaggerated neutrophilic inflammation and defective IL-10 production in thbs1 −/− mice following lipopolysaccharide (LPS)-induced lung injury. In contrast to previous report, 14 we did not ... All experimental procedures with animals were approved by the Institutional Animal Care and Use Committee (IACUC) of Cincinnati Children’s Medical Center, protocols IACUC 2016-0069 and 2024-0047. The number of mice used in this study reflects the minimum number needed to achieve statistical significance based on … Se mer Full-length mouse Atf4 cDNA was obtained from Addgene (plasmid #21845, deposited by Dr. David Ron), amplified by PCR using CloneAMP HiFi PCR premix (Takara Bio, #639298) and cloned into the Cla1 and XhoI sites of … Se mer Adenoviruses harboring Thbs1, Thbs3, tandem mRFP-GFP-LC3 (Ad-tf-LC3; gift from Dr. J. Sadoshima of the University of Medicine and Dentistry of New Jersey, Newark), and β-galactosidase (βgal) expressing control were … Se mer Hearts were excised, immediately frozen in liquid nitrogen and stored at −80 °C until further use. Cultured primary NRVMs were washed three times … Se mer Protein extracts from tTA cont. and Thbs1 DTG hearts were generated by homogenizing in immunoprecipitation (IP) lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% Triton X … Se mer
NettetA cDNA ORF corresponding to the peptide of Mouse Thrombospondin-1/Thbs1 was expressed with an N-terminal 6xHis-tag in E.coli. Thrombospondin-1/Thbs1 CSB-EP023487MO is a truncated molecule having amino acids of Asn19-Lys350. Nettet3. jan. 2024 · A stratified transcriptomics analysis of polygenic fat and lean mouse adipose tissues identifies Thbs1 as novel candidate obesity genes. The effect of blockade of …
NettetHow it's Used. Nettet30. mai 2024 · Abstract In this study, the effect of the THBS1 gene knockout on the survival of human tumor cells, the frequency of spontaneous and radiation-induced micronuclei, and the expression profile of genes in the HeLa cell line was investigated. It was shown that the THBS1 gene knockout led to a decrease in the plating efficiency …
NettetLS-F4336 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse THBS1 / Thrombospondin-1 in samples of Plasma and Tissue …
Nettet5. apr. 2024 · THBS1 facilitates colorectal liver metastasis through enhancing epithelial-mesenchymal transition. Thrombospondin 1 improves hepatic steatosis in diet-induced insulin-resistant mice and is associated with hepatic fat content in humans. Thrombospondin-1 CD47 Signalling: From Mechanisms to Medicine. ear wax drops stuck in earNettet1. okt. 2024 · Increased THBS1 levels are associated with aging during which the ECM becomes stiffer, whereas mice lacking THBS1 show reduced collagen crosslinking in skin (14). We observed increased expression and deposition of THBS1 in the ECM of nonarticular cartilage of CreTW mice, which was associated with an increase in … ctsh rcepNettetPhenotype data for mouse gene Thbs1. Discover Thbs1's significant phenotypes, expression, images, histopathology and more. Data for gene Thbs1 is all freely … ear wax falling out of ear redditNettet11. jul. 2014 · By in situ hybridization, we detected a 6.73-fold increase in the number of Thbs1 mRNA/GFAP-expressing ACs (Fig. 7a–c) in the WT mice when compared with … ctsh + rvc 35%Nettet2. sep. 2016 · Here we show a critical novel role for thrombospondin 1 (THBS1) in β-cell survival during lipotoxic stress in rat, mouse and human models. THBS1 acts from within the endoplasmic reticulum to ... ctsh q4 2021 resultsNettet1. des. 2013 · Thbs1 expression during adipogenesis and HFD-induced obesity. A, Thbs1 expression during the differentiation of 3T3-L1 cells into adipocytes (n = 3, mean ± SEM). The induction of aP2 (Fabp4) and Pparγ is shown for comparison.B, Thbs1 expression in the iWAT, eWAT, and brown adipose tissues isolated from 11-week-old C57BL/6J male … ct shredderNettet21. apr. 2024 · Thrombospondin-1 (TSP1) is a matricellular protein that negatively regulates angiogenesis and inflammation in the eye. It aids ocular vascular … ear wax falling out my ear