WebApr 14, 2024 · Notch-RBP-J signaling plays an essential role in maintenance of myeloid homeostasis. However, its role in monocyte cell fate decisions is not fully understood. Here we showed that conditional deletion of transcription factor RBP-J in myeloid cells resulted in marked accumulation of blood Ly6Clo monocytes that highly expressed chemokine … WebOct 9, 2024 · The Notch signal transduction cascade requires cell-to-cell contact and results in the proteolytic processing of the Notch receptor and subsequent assembly of a transcriptional coactivator complex containing the Notch intracellular domain (NICD) and transcription factor RBPJ. In the absence of a Notch signal, RBPJ remains at Notch target …
RBP-J regulates homeostasis and function of circulating Ly6C lo ...
WebFeb 17, 2011 · The Notch signaling pathway plays a pivotal role in the maintenance of stem/progenitor cells and the regulation of glial versus neuronal identity. To study the effect of Notch signaling on Hh-activated neural progenitors, we inactivated both Patched1 and Rbpj, a transcriptional mediator of Notch signaling, in Nestin positive cells of the neocortex. WebFeb 28, 2016 · RBPJ forms a complex with the NICD that is stabilized by MAML3, and Notch signaling is activated by binding of the RBPJ/NICD/MAML3 complex to DNA in the nucleus [14]. Indeed, the expression of Hes1-3 that is a Notch signaling target gene was suppressed by RBPJ and MAML3 inhibition ( Supplementary Fig. S1 ), suggesting that RBPJ and … incoming quality
IJMS Free Full-Text The Binding of CSL Proteins to Either Co ...
WebJun 30, 2016 · RBPJ is ubiquitously expressed, and can either activate genes by forming a complex with the Notch intracellular domain (NICD) when Notch is active 10, or silence an … WebRBPJ occupies a limited number of sites in mouse myogenic cells. To identify RBPJ-binding sites under different Notch signaling conditions, we performed ChIP-seq in mouse myogenic cells (C2C12) for targets of RBPJ and also of p300, a HAT that marks enhancer regulatory elements (Visel et al. 2009) and, together with NICD, acts as a coactivator of RBPJ … WebApr 19, 2024 · Loss of endothelial Notch signaling induces spontaneous formation of TLSs. To study the role of Notch signaling in vascular and immune homeostasis in the kidney, we generated Cdh5Cre ERT2;Rbpj fl ... incoming proprietor pexa