Texas red phalloidin
Web40 rows · A toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. When conjugated to a fluorescent label, it will stain actin filaments in fixed and permeabilized … WebRhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates, as evidenced by over 19,000 citations in the literature. We offer rhodamine reactive dyes …
Texas red phalloidin
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WebPrepare a 1 mL solution containing 50 to 100 µg/mL lysopalmitoylphosphatidylcholine and 3.7% formaldehyde and then add 5–10 units of fluorescent phallotoxin (approximately 25 … WebTexas Red™-X phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Texas Red™-X phalloidin staining is fully … Texas Red™-X phalloidin is a high-affinity F-actin probe conjugated to our bright, … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
WebOct 1, 1999 · For visualization of intracellular F-actin organization, the cells were probed with Texas Red-conjugated phalloidin. For detection of nontagged CPD, the samples were incubated with the anti-CPD antibody, followed by an FITC-conjugated secondary antibody. Fluorescent images were visualized using a confocal microscope (MRC1024; Bio-Rad). … WebPhalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom. This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it …
WebPhalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin, and this was first identified in phalloidin … WebMay 20, 2024 · DAPI (1:2000) was applied for nuclear staining and Texas Red Phalloidin (1:400) was applied for the staining of actin at the same time as the secondary antibody. Cells were imaged and the colocalization of the 67LR with actin was analyzed on a Leica TCS SP8 confocal microscope with the same settings that were described in the previous …
WebTexas Red X Phalloidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol …
WebPhalloidin is a bicyclic heptapeptide isolated from the poisonous death cap mushroom, Amanita phalloides.Its high binding affinity for the grooves between filamentous actin over monomeric G-actin is widely used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations.Compared to actin-specific antibodies, the non-specific … sneg mathematicaWebOct 13, 2024 · Texas Red-phalloidin and Hoechst 33342 from Molecular Probes (Eugene, OR) were used to label F-actin and nuclei, respectively. Monoclonal antibodies against the HA (Covance) or GM130 (BD Transduction Laboratories) were used, followed by incubation with fluorescein isothiocyanate-donkey anti-mouse antibody (Jackson ImmunoResearch … snegithiye songsWebThe green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution). road trip to niagara fallsWebDec 5, 2003 · Endogenous RhoA staining was visualized with an anti-RhoA primary antibody (green), and actin was detected with Texas red–conjugated phalloidin (red). Superimposition of the images (merge) reveals colocalization of RhoA and F-actin in the protrusions of Smurf1 siRNA-treated HEK293T cells (yellow arrowheads). sneha acharya and ryanWebAdd Phalloidin-iFluor™ 594 Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under microscope with TRITC or Texas Red filter Important Warm the vial to room temperature and centrifuge briefly before opening. Storage and Handling Conditions road trip to niagara falls canadaWebOct 2, 2024 · F-actin was visualized by Texas Red-phalloidin. Data are representative images of three independent experiments (a, c, and e), and >25 cells were analyzed in three independent experiments (b, d, and f). (g and h) Activities of CDC42, RAC1, and RAC2. Activities of CDC42, RAC1, and RAC2 were measured by GST-PBD pulldown coupled with … sneha acharyaWebDemonstrating very little nonspecific staining, Texas Red™-X phalloidin allows high-contrast discrimination of actin staining. Use in Multiple Applications Texas Red™-X phalloidin … sneglehuset thyborøn facebook